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Corning Life Sciences 6w ula plates
6w Ula Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/6w+plate/6w+ula+plates/pm39827811-68-8-11
Average 90 stars, based on 1 article reviews
6w ula plates - by Bioz Stars, 2026-10
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Article Title: Geometric effects position renal vesicles during kidney development.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-LIM1/LHX1 abcam Cat#ab229474; RRID:AB_2924798 Anti-LIM1/LHX1 DSHB DSHB Hybridoma Product 4F2; RRID:AB_531784 Anti-SIX2 proteintech Cat#11562-1-AP; RRID:AB_2189084 Chemicals, peptides, and recombinant proteins WNT9B R&D Cat#3669-WN Tamoxifen Thermo Scientific Chemicals Cat#J63509.03 DMEM-F12 Gibco Cat#21041025 trypLE Gibco Cat#12604013 FBS Gibco Cat#A5256801 EDTA Thermo Scientific Chemicals Cat#17892 DMEM Gibco Cat#41965039 DMSO Thermo Scientific Chemicals Cat#J66650.AK SYBR Green SuperMix Bio-Rad Cat#1725120 CHIR99021 Stemcell technologies Cat#72052 BSA Sigma-Aldrich Cat#A7906-10G Critical commercial assays Absolutely Rna Nanoperp kit Agilent technologies Cat#400753-12 PrimeScript RT Reagent Kit Takara Cat#RR037B Deposited data Source code This Paper https://git.bsse.ethz.ch/iber/Publications/ 2022_mederacke_conrad_renal_vesicles COMSOL model files This Paper https://git.bsse.ethz.ch/iber/Publications/ 2022_mederacke_conrad_renal_vesicles Experimental models: Organisms/strains Mouse: RjOrl:SWISS N/A RjOrl:SWISS Mouse: Tg(Hoxb7-Venus*)17Cos Chi et al.57 RRID:IMSR_JAX:016252 Mouse: B6; 129-Six2tm3(EGFP/cre/ERT2)Amc/J Kobayash et al.58 RRID:IMSR_JAX:009600 Mouse: B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J Madisen et al.59 RRID:IMSR_JAX:007914 Oligonucleotides mouse gapdh forward: AACTTTGGCATTGTGGAAGG This paper N/A mouse gapdh reverse: ATCCACAGTCTTCTGGGTGG This paper N/A mouse pax 8 forward: CACAAAGGCCCCTCCTAGTT This paper N/A mouse pax 8 reverse: GCGAGTGTCCCTCAGTCTGT This paper N/A mouse wnt 4 forward: CTCAAAGGCCTGATCCAGAG This paper N/A mouse wnt 4 reverse: TCACAGCCACACTTCTCCAG This paper N/A mouse cited 1 forward: CATCCTTCAACCTGCATCCT This paper N/A mouse cited 1 reverse: ACCAGCAGGAGGAGAGACAG This paper N/A mouse lef1 forward: TCATCACCTACAGCGACGAG This paper N/A mouse lef1 reverse: GAAGGTGGGGATTTCAGGAG This paper N/A (Continued on next page) Cell Reports 42, 113526, December 26, 2023 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms ImageJ https://imagej.nih.gov/ij/index.html COMSOL 6.0 COMSOL Multiphysics http://comsol.com Python 3.9 Python https://www.python.org MATLAB 2022b Mathworks https://www.mathworks.com R 4.2.1 R Project https://www.r-project.org Blender 3.0 Blender https://www.blender.org Imaris 10.0 Bitplane, Oxford Instruments https://imaris.oxinst.com QuantStudio 3 Thermo Fisher Scientific Other Affi-Gel Blue beads Biorad Cat#153-730 96w Plate Corning Cat#3598 6w Plate Corning Cat3516 24 mm Transwell with 0.4 mm Pore Polyester Membrane Insert Corning Cat#3450 ..

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Article Title: Geometric effects position renal vesicles during kidney development.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-LIM1/LHX1 abcam Cat#ab229474; RRID:AB_2924798 Anti-LIM1/LHX1 DSHB DSHB Hybridoma Product 4F2; RRID:AB_531784 Anti-SIX2 proteintech Cat#11562-1-AP; RRID:AB_2189084 Chemicals, peptides, and recombinant proteins WNT9B R&D Cat#3669-WN Tamoxifen Thermo Scientific Chemicals Cat#J63509.03 DMEM-F12 Gibco Cat#21041025 trypLE Gibco Cat#12604013 FBS Gibco Cat#A5256801 EDTA Thermo Scientific Chemicals Cat#17892 DMEM Gibco Cat#41965039 DMSO Thermo Scientific Chemicals Cat#J66650.AK SYBR Green SuperMix Bio-Rad Cat#1725120 CHIR99021 Stemcell technologies Cat#72052 BSA Sigma-Aldrich Cat#A7906-10G Critical commercial assays Absolutely Rna Nanoperp kit Agilent technologies Cat#400753-12 PrimeScript RT Reagent Kit Takara Cat#RR037B Deposited data Source code This Paper https://git.bsse.ethz.ch/iber/Publications/ 2022_mederacke_conrad_renal_vesicles COMSOL model files This Paper https://git.bsse.ethz.ch/iber/Publications/ 2022_mederacke_conrad_renal_vesicles Experimental models: Organisms/strains Mouse: RjOrl:SWISS N/A RjOrl:SWISS Mouse: Tg(Hoxb7-Venus*)17Cos Chi et al.57 RRID:IMSR_JAX:016252 Mouse: B6; 129-Six2tm3(EGFP/cre/ERT2)Amc/J Kobayash et al.58 RRID:IMSR_JAX:009600 Mouse: B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J Madisen et al.59 RRID:IMSR_JAX:007914 Oligonucleotides mouse gapdh forward: AACTTTGGCATTGTGGAAGG This paper N/A mouse gapdh reverse: ATCCACAGTCTTCTGGGTGG This paper N/A mouse pax 8 forward: CACAAAGGCCCCTCCTAGTT This paper N/A mouse pax 8 reverse: GCGAGTGTCCCTCAGTCTGT This paper N/A mouse wnt 4 forward: CTCAAAGGCCTGATCCAGAG This paper N/A mouse wnt 4 reverse: TCACAGCCACACTTCTCCAG This paper N/A mouse cited 1 forward: CATCCTTCAACCTGCATCCT This paper N/A mouse cited 1 reverse: ACCAGCAGGAGGAGAGACAG This paper N/A mouse lef1 forward: TCATCACCTACAGCGACGAG This paper N/A mouse lef1 reverse: GAAGGTGGGGATTTCAGGAG This paper N/A (Continued on next page) Cell Reports 42, 113526, December 26, 2023 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms ImageJ https://imagej.nih.gov/ij/index.html COMSOL 6.0 COMSOL Multiphysics http://comsol.com Python 3.9 Python https://www.python.org MATLAB 2022b Mathworks https://www.mathworks.com R 4.2.1 R Project https://www.r-project.org Blender 3.0 Blender https://www.blender.org Imaris 10.0 Bitplane, Oxford Instruments https://imaris.oxinst.com QuantStudio 3 Thermo Fisher Scientific Other Affi-Gel Blue beads Biorad Cat#153-730 96w Plate Corning Cat#3598 6w Plate Corning Cat3516 24 mm Transwell with 0.4 mm Pore Polyester Membrane Insert Corning Cat#3450 ..



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A Representative images of sections from the day-60 <t>cerebral</t> <t>organoids</t> stained with SYN1 and MAP2. Scale bar, 20 μm. B Quantification of SYN1 + puncta density in both the isogenic control (Correct) and the patient-derived (Patient) organoids on day 60. N = 12. C Representative bright-field image of a day-45 organoid plated on a 64-electrode array in an <t>MEA</t> well. D Plate maps of mean firing rates of both Correct and Patient organoids on day 45 and day 60. E Representative wave forms of spiking behavior from a single electrode for each Correct or Patient organoid on day 60. F Representative raster plots of Correct and Patient organoids (day 60) over 5-min continuous recording. Quantification of mean firing rate ( G ), number of spikes ( H ), burst percentage ( I ) and burst frequency ( J ) based on data obtained from Correct and Patient organoids on day 45 and day 60 over 10 min recording. A bundle of more than 10 spikes within 100 ms was defined as a burst. N = 8 MEA wells per genotype. Data are presented as the mean ± SEM. The statistical significance was determined using two-tailed unpaired t-test. * P < 0.05; ** P < 0.01; *** P < 0.001. ns: no significance.
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A Representative images of sections from the day-60 <t>cerebral</t> <t>organoids</t> stained with SYN1 and MAP2. Scale bar, 20 μm. B Quantification of SYN1 + puncta density in both the isogenic control (Correct) and the patient-derived (Patient) organoids on day 60. N = 12. C Representative bright-field image of a day-45 organoid plated on a 64-electrode array in an <t>MEA</t> well. D Plate maps of mean firing rates of both Correct and Patient organoids on day 45 and day 60. E Representative wave forms of spiking behavior from a single electrode for each Correct or Patient organoid on day 60. F Representative raster plots of Correct and Patient organoids (day 60) over 5-min continuous recording. Quantification of mean firing rate ( G ), number of spikes ( H ), burst percentage ( I ) and burst frequency ( J ) based on data obtained from Correct and Patient organoids on day 45 and day 60 over 10 min recording. A bundle of more than 10 spikes within 100 ms was defined as a burst. N = 8 MEA wells per genotype. Data are presented as the mean ± SEM. The statistical significance was determined using two-tailed unpaired t-test. * P < 0.05; ** P < 0.01; *** P < 0.001. ns: no significance.
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A Representative images of sections from the day-60 <t>cerebral</t> <t>organoids</t> stained with SYN1 and MAP2. Scale bar, 20 μm. B Quantification of SYN1 + puncta density in both the isogenic control (Correct) and the patient-derived (Patient) organoids on day 60. N = 12. C Representative bright-field image of a day-45 organoid plated on a 64-electrode array in an <t>MEA</t> well. D Plate maps of mean firing rates of both Correct and Patient organoids on day 45 and day 60. E Representative wave forms of spiking behavior from a single electrode for each Correct or Patient organoid on day 60. F Representative raster plots of Correct and Patient organoids (day 60) over 5-min continuous recording. Quantification of mean firing rate ( G ), number of spikes ( H ), burst percentage ( I ) and burst frequency ( J ) based on data obtained from Correct and Patient organoids on day 45 and day 60 over 10 min recording. A bundle of more than 10 spikes within 100 ms was defined as a burst. N = 8 MEA wells per genotype. Data are presented as the mean ± SEM. The statistical significance was determined using two-tailed unpaired t-test. * P < 0.05; ** P < 0.01; *** P < 0.001. ns: no significance.
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A Representative images of sections from the day-60 <t>cerebral</t> <t>organoids</t> stained with SYN1 and MAP2. Scale bar, 20 μm. B Quantification of SYN1 + puncta density in both the isogenic control (Correct) and the patient-derived (Patient) organoids on day 60. N = 12. C Representative bright-field image of a day-45 organoid plated on a 64-electrode array in an <t>MEA</t> well. D Plate maps of mean firing rates of both Correct and Patient organoids on day 45 and day 60. E Representative wave forms of spiking behavior from a single electrode for each Correct or Patient organoid on day 60. F Representative raster plots of Correct and Patient organoids (day 60) over 5-min continuous recording. Quantification of mean firing rate ( G ), number of spikes ( H ), burst percentage ( I ) and burst frequency ( J ) based on data obtained from Correct and Patient organoids on day 45 and day 60 over 10 min recording. A bundle of more than 10 spikes within 100 ms was defined as a burst. N = 8 MEA wells per genotype. Data are presented as the mean ± SEM. The statistical significance was determined using two-tailed unpaired t-test. * P < 0.05; ** P < 0.01; *** P < 0.001. ns: no significance.
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A Representative images of sections from the day-60 <t>cerebral</t> <t>organoids</t> stained with SYN1 and MAP2. Scale bar, 20 μm. B Quantification of SYN1 + puncta density in both the isogenic control (Correct) and the patient-derived (Patient) organoids on day 60. N = 12. C Representative bright-field image of a day-45 organoid plated on a 64-electrode array in an <t>MEA</t> well. D Plate maps of mean firing rates of both Correct and Patient organoids on day 45 and day 60. E Representative wave forms of spiking behavior from a single electrode for each Correct or Patient organoid on day 60. F Representative raster plots of Correct and Patient organoids (day 60) over 5-min continuous recording. Quantification of mean firing rate ( G ), number of spikes ( H ), burst percentage ( I ) and burst frequency ( J ) based on data obtained from Correct and Patient organoids on day 45 and day 60 over 10 min recording. A bundle of more than 10 spikes within 100 ms was defined as a burst. N = 8 MEA wells per genotype. Data are presented as the mean ± SEM. The statistical significance was determined using two-tailed unpaired t-test. * P < 0.05; ** P < 0.01; *** P < 0.001. ns: no significance.
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A Representative images of sections from the day-60 cerebral organoids stained with SYN1 and MAP2. Scale bar, 20 μm. B Quantification of SYN1 + puncta density in both the isogenic control (Correct) and the patient-derived (Patient) organoids on day 60. N = 12. C Representative bright-field image of a day-45 organoid plated on a 64-electrode array in an MEA well. D Plate maps of mean firing rates of both Correct and Patient organoids on day 45 and day 60. E Representative wave forms of spiking behavior from a single electrode for each Correct or Patient organoid on day 60. F Representative raster plots of Correct and Patient organoids (day 60) over 5-min continuous recording. Quantification of mean firing rate ( G ), number of spikes ( H ), burst percentage ( I ) and burst frequency ( J ) based on data obtained from Correct and Patient organoids on day 45 and day 60 over 10 min recording. A bundle of more than 10 spikes within 100 ms was defined as a burst. N = 8 MEA wells per genotype. Data are presented as the mean ± SEM. The statistical significance was determined using two-tailed unpaired t-test. * P < 0.05; ** P < 0.01; *** P < 0.001. ns: no significance.

Journal: Cell Death & Disease

Article Title: CUL4B mutations impair human cortical neurogenesis through PP2A-dependent inhibition of AKT and ERK

doi: 10.1038/s41419-024-06501-3

Figure Lengend Snippet: A Representative images of sections from the day-60 cerebral organoids stained with SYN1 and MAP2. Scale bar, 20 μm. B Quantification of SYN1 + puncta density in both the isogenic control (Correct) and the patient-derived (Patient) organoids on day 60. N = 12. C Representative bright-field image of a day-45 organoid plated on a 64-electrode array in an MEA well. D Plate maps of mean firing rates of both Correct and Patient organoids on day 45 and day 60. E Representative wave forms of spiking behavior from a single electrode for each Correct or Patient organoid on day 60. F Representative raster plots of Correct and Patient organoids (day 60) over 5-min continuous recording. Quantification of mean firing rate ( G ), number of spikes ( H ), burst percentage ( I ) and burst frequency ( J ) based on data obtained from Correct and Patient organoids on day 45 and day 60 over 10 min recording. A bundle of more than 10 spikes within 100 ms was defined as a burst. N = 8 MEA wells per genotype. Data are presented as the mean ± SEM. The statistical significance was determined using two-tailed unpaired t-test. * P < 0.05; ** P < 0.01; *** P < 0.001. ns: no significance.

Article Snippet: Organoids were placed into 6-well MEA plates (Axion Biosystems, Atlanta, GA, USA, Cat# M384-tMEA-6W) coated with poly-l-ornithine/laminin (Sigma) and with 64 platinum microelectrodes arrays per well.

Techniques: Staining, Control, Derivative Assay, Two Tailed Test